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Image Search Results
Journal: bioRxiv
Article Title: PTGER2-β-Catenin Axis Links High Salt Environments to Autoimmunity by Balancing IFNγ and IL-10 in FoxP3 + Regulatory T cells
doi: 10.1101/379453
Figure Lengend Snippet: (a) Relative expression level of Active β-catenin (ABC) on ex vivo Treg subpopulations analyzed by flow cytometry (n=11 subjects). Fold change in gMFI over DN were depicted. * P <0.05, ** P <0.01 (one-way ANOVA with Tukey’s multiple comparisons test). gMFI, geometric mean fluorescence intensity. (b) Expression level of ABC between CXCR3 − and CXCR3 + ex vivo Tregs from healthy controls. Representative histogram (left) and summary of results (n=7 subjects) (right). * P <0.05 (two-tailed paired Student’s t -test). (c) Gene expression of Wnt/ β-catenin signaling target genes ( AXIN2 and TCF7 ) assessed by RNA-seq. * P <0.05, ** P <0.01 (one-way ANOVA with Tukey’s multiple comparisons test). (d) Relative expression level of ABC on Tregs stimulated with anti-CD3 and anti-CD28 for 4 days, followed by 4 h PMA/iomomycin stimulation and intracellular cytokine staining for IFNγ and IL-10 (n=12 subjects). Fold change in gMFI over DN were depicted. * P <0.05, ** P <0.01, *** P <0.001 (one-way ANOVA with Tukey’s multiple comparisons test). (e) Expression level of β-catenin on Tregs stimulated with anti-CD3 and anti-CD28 in the presence (Th1) or absence (Th0) of IL-12 for 4 days. β-catenin level was determined directly by intracellular staining (left) (n=9 subjects) and β-catenin level on IFNγ positive/negative Treg populations was determined after 4 h PMA/iomomycin stimulation (middle) (n=4 subjects). Representative histogram for β-catenin expression was shown (right). * P <0.05 (two-tailed unpaired Student’s t -test). (f, g) Frequency of IFNγ and IL-10 positive cell number ( f ) and gene expression of IFNG and IL10 by qPCR ( g ). Tregs were stimulated with anti-CD3 and anti-CD28 in the presence of Wnt/β-catenin signaling inhibitor PKF115-584 (PKF), IL-12 (Th1), or IL-12 and PKF115-584 (Th1 + PKF) (n=4 subjects) * P <0.05, ** P <0.01, *** P <0.001 (one-way ANOVA with Tukey’s multiple comparisons test). (h, i) Relative frequency of IFNγ and IL-10 positive cell number (fold of scramble shRNA/control condition) ( h ) and gene expression of IFNG and IL10 by qPCR ( i ). Tregs were transduced with a non-targeted shRNA or a CTNNB1 shRNA and cultured in Th0 or Th1 condition for 5 days ( h ; n=7 subjects, i ; n=5 subjects). * P <0.05, ** P <0.01 (one-way ANOVA with Tukey’s multiple comparisons test). Data are representative of two experiments (e (middle), and f) or are from more than three experiments.
Article Snippet: Th1-Tregs induced with human
Techniques: Expressing, Ex Vivo, Flow Cytometry, Fluorescence, Two Tailed Test, RNA Sequencing Assay, Staining, shRNA, Transduction, Cell Culture
Journal: bioRxiv
Article Title: PTGER2-β-Catenin Axis Links High Salt Environments to Autoimmunity by Balancing IFNγ and IL-10 in FoxP3 + Regulatory T cells
doi: 10.1101/379453
Figure Lengend Snippet: (a) AXIN2 and TCF7 mRNA expression in IFNγ + and IFNγ − human Treg populations assessed by DNA microarray (n=8 subjects) . * P <0.05 (two-tailed paired Student’s t -test). (b) CTNNB1 gene expression on Tregs transduced with a non-targeted shRNA or a CTNNB1 shRNA and cultured for 5 days (n=10 subjects). *** P <0.001 (two-tailed unpaired Student’s t -test). (c) Flow cytometric analysis of TCF1 expression on ex vivo Treg subpopulations relative to DN (n=8 subjects). * P <0.05, ** P <0.01 (one-way ANOVA with Tukey’s multiple comparisons test). (d) Frequency of IFNγ and IL-10 positive cell number. Tregs were stimulated with anti-CD3 and anti-CD28 in the presence of SGK1 inhibitor GSK650394 (SGK1-i), IL-12 (Th1), or IL-12 and GSK650394 (Th1 + SGK1-i) (n=6 subjects) *** P <0.001 (one-way ANOVA with Tukey’s multiple comparisons test).
Article Snippet: Th1-Tregs induced with human
Techniques: Expressing, Microarray, Two Tailed Test, Transduction, shRNA, Cell Culture, Ex Vivo
Journal: Bioscience Reports
Article Title: Differentially expressed circulating miRNAs in postmenopausal osteoporosis: a meta-analysis
doi: 10.1042/BSR20190667
Figure Lengend Snippet: Characteristics of included miRNA profiling studies
Article Snippet: 12 , Chen C , 2013 , China , Blood , 20 (10/10) ,
Techniques: Clinical Proteomics, Microarray, Sequencing
Journal: Bioscience Reports
Article Title: Differentially expressed circulating miRNAs in postmenopausal osteoporosis: a meta-analysis
doi: 10.1042/BSR20190667
Figure Lengend Snippet: * indicates miRNAs reported in more than one study, # indicates miRNAs reported to be dysregulated in both directions.
Article Snippet: 12 , Chen C , 2013 , China , Blood , 20 (10/10) ,
Techniques: