phenotype microarray bacterial chemical sensitivity assay panels 12 – 20 Search Results


96
R&D Systems recombinant il 12
(a) Relative expression level of Active β-catenin (ABC) on ex vivo Treg subpopulations analyzed by flow cytometry (n=11 subjects). Fold change in gMFI over DN were depicted. * P <0.05, ** P <0.01 (one-way ANOVA with Tukey’s multiple comparisons test). gMFI, geometric mean fluorescence intensity. (b) Expression level of ABC between CXCR3 − and CXCR3 + ex vivo Tregs from healthy controls. Representative histogram (left) and summary of results (n=7 subjects) (right). * P <0.05 (two-tailed paired Student’s t -test). (c) Gene expression of Wnt/ β-catenin signaling target genes ( AXIN2 and TCF7 ) assessed by RNA-seq. * P <0.05, ** P <0.01 (one-way ANOVA with Tukey’s multiple comparisons test). (d) Relative expression level of ABC on Tregs stimulated with anti-CD3 and anti-CD28 for 4 days, followed by 4 h PMA/iomomycin stimulation and intracellular cytokine staining for IFNγ and IL-10 (n=12 subjects). Fold change in gMFI over DN were depicted. * P <0.05, ** P <0.01, *** P <0.001 (one-way ANOVA with Tukey’s multiple comparisons test). (e) Expression level of β-catenin on Tregs stimulated with anti-CD3 and anti-CD28 in the presence (Th1) or absence (Th0) of <t>IL-12</t> for 4 days. β-catenin level was determined directly by intracellular staining (left) (n=9 subjects) and β-catenin level on IFNγ positive/negative Treg populations was determined after 4 h PMA/iomomycin stimulation (middle) (n=4 subjects). Representative histogram for β-catenin expression was shown (right). * P <0.05 (two-tailed unpaired Student’s t -test). (f, g) Frequency of IFNγ and IL-10 positive cell number ( f ) and gene expression of IFNG and IL10 by qPCR ( g ). Tregs were stimulated with anti-CD3 and anti-CD28 in the presence of Wnt/β-catenin signaling inhibitor PKF115-584 (PKF), IL-12 (Th1), or IL-12 and PKF115-584 (Th1 + PKF) (n=4 subjects) * P <0.05, ** P <0.01, *** P <0.001 (one-way ANOVA with Tukey’s multiple comparisons test). (h, i) Relative frequency of IFNγ and IL-10 positive cell number (fold of scramble shRNA/control condition) ( h ) and gene expression of IFNG and IL10 by qPCR ( i ). Tregs were transduced with a non-targeted shRNA or a CTNNB1 shRNA and cultured in Th0 or Th1 condition for 5 days ( h ; n=7 subjects, i ; n=5 subjects). * P <0.05, ** P <0.01 (one-way ANOVA with Tukey’s multiple comparisons test). Data are representative of two experiments (e (middle), and f) or are from more than three experiments.
Recombinant Il 12, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phenotype+microarray+bacterial+chemical+sensitivity+assay+panels+12+%E2%80%93+20/Recombinant+Human+IL-12+Protein/bio_rxiv__379453-236-4-8
Average 96 stars, based on 1 article reviews
recombinant il 12 - by Bioz Stars, 2026-09
96/100 stars
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99
PCR Biosystems Ltd qpcrbio sygreen mix pcr biosystems
(a) Relative expression level of Active β-catenin (ABC) on ex vivo Treg subpopulations analyzed by flow cytometry (n=11 subjects). Fold change in gMFI over DN were depicted. * P <0.05, ** P <0.01 (one-way ANOVA with Tukey’s multiple comparisons test). gMFI, geometric mean fluorescence intensity. (b) Expression level of ABC between CXCR3 − and CXCR3 + ex vivo Tregs from healthy controls. Representative histogram (left) and summary of results (n=7 subjects) (right). * P <0.05 (two-tailed paired Student’s t -test). (c) Gene expression of Wnt/ β-catenin signaling target genes ( AXIN2 and TCF7 ) assessed by RNA-seq. * P <0.05, ** P <0.01 (one-way ANOVA with Tukey’s multiple comparisons test). (d) Relative expression level of ABC on Tregs stimulated with anti-CD3 and anti-CD28 for 4 days, followed by 4 h PMA/iomomycin stimulation and intracellular cytokine staining for IFNγ and IL-10 (n=12 subjects). Fold change in gMFI over DN were depicted. * P <0.05, ** P <0.01, *** P <0.001 (one-way ANOVA with Tukey’s multiple comparisons test). (e) Expression level of β-catenin on Tregs stimulated with anti-CD3 and anti-CD28 in the presence (Th1) or absence (Th0) of <t>IL-12</t> for 4 days. β-catenin level was determined directly by intracellular staining (left) (n=9 subjects) and β-catenin level on IFNγ positive/negative Treg populations was determined after 4 h PMA/iomomycin stimulation (middle) (n=4 subjects). Representative histogram for β-catenin expression was shown (right). * P <0.05 (two-tailed unpaired Student’s t -test). (f, g) Frequency of IFNγ and IL-10 positive cell number ( f ) and gene expression of IFNG and IL10 by qPCR ( g ). Tregs were stimulated with anti-CD3 and anti-CD28 in the presence of Wnt/β-catenin signaling inhibitor PKF115-584 (PKF), IL-12 (Th1), or IL-12 and PKF115-584 (Th1 + PKF) (n=4 subjects) * P <0.05, ** P <0.01, *** P <0.001 (one-way ANOVA with Tukey’s multiple comparisons test). (h, i) Relative frequency of IFNγ and IL-10 positive cell number (fold of scramble shRNA/control condition) ( h ) and gene expression of IFNG and IL10 by qPCR ( i ). Tregs were transduced with a non-targeted shRNA or a CTNNB1 shRNA and cultured in Th0 or Th1 condition for 5 days ( h ; n=7 subjects, i ; n=5 subjects). * P <0.05, ** P <0.01 (one-way ANOVA with Tukey’s multiple comparisons test). Data are representative of two experiments (e (middle), and f) or are from more than three experiments.
Qpcrbio Sygreen Mix Pcr Biosystems, supplied by PCR Biosystems Ltd, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
qpcrbio sygreen mix pcr biosystems - by Bioz Stars, 2026-09
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90
YorkTest Laboratories genarrayt microarray 200+ food igg
(a) Relative expression level of Active β-catenin (ABC) on ex vivo Treg subpopulations analyzed by flow cytometry (n=11 subjects). Fold change in gMFI over DN were depicted. * P <0.05, ** P <0.01 (one-way ANOVA with Tukey’s multiple comparisons test). gMFI, geometric mean fluorescence intensity. (b) Expression level of ABC between CXCR3 − and CXCR3 + ex vivo Tregs from healthy controls. Representative histogram (left) and summary of results (n=7 subjects) (right). * P <0.05 (two-tailed paired Student’s t -test). (c) Gene expression of Wnt/ β-catenin signaling target genes ( AXIN2 and TCF7 ) assessed by RNA-seq. * P <0.05, ** P <0.01 (one-way ANOVA with Tukey’s multiple comparisons test). (d) Relative expression level of ABC on Tregs stimulated with anti-CD3 and anti-CD28 for 4 days, followed by 4 h PMA/iomomycin stimulation and intracellular cytokine staining for IFNγ and IL-10 (n=12 subjects). Fold change in gMFI over DN were depicted. * P <0.05, ** P <0.01, *** P <0.001 (one-way ANOVA with Tukey’s multiple comparisons test). (e) Expression level of β-catenin on Tregs stimulated with anti-CD3 and anti-CD28 in the presence (Th1) or absence (Th0) of <t>IL-12</t> for 4 days. β-catenin level was determined directly by intracellular staining (left) (n=9 subjects) and β-catenin level on IFNγ positive/negative Treg populations was determined after 4 h PMA/iomomycin stimulation (middle) (n=4 subjects). Representative histogram for β-catenin expression was shown (right). * P <0.05 (two-tailed unpaired Student’s t -test). (f, g) Frequency of IFNγ and IL-10 positive cell number ( f ) and gene expression of IFNG and IL10 by qPCR ( g ). Tregs were stimulated with anti-CD3 and anti-CD28 in the presence of Wnt/β-catenin signaling inhibitor PKF115-584 (PKF), IL-12 (Th1), or IL-12 and PKF115-584 (Th1 + PKF) (n=4 subjects) * P <0.05, ** P <0.01, *** P <0.001 (one-way ANOVA with Tukey’s multiple comparisons test). (h, i) Relative frequency of IFNγ and IL-10 positive cell number (fold of scramble shRNA/control condition) ( h ) and gene expression of IFNG and IL10 by qPCR ( i ). Tregs were transduced with a non-targeted shRNA or a CTNNB1 shRNA and cultured in Th0 or Th1 condition for 5 days ( h ; n=7 subjects, i ; n=5 subjects). * P <0.05, ** P <0.01 (one-way ANOVA with Tukey’s multiple comparisons test). Data are representative of two experiments (e (middle), and f) or are from more than three experiments.
Genarrayt Microarray 200+ Food Igg, supplied by YorkTest Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
genarrayt microarray 200+ food igg - by Bioz Stars, 2026-09
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90
INFINIUM Inc infinium genotyping assays
(a) Relative expression level of Active β-catenin (ABC) on ex vivo Treg subpopulations analyzed by flow cytometry (n=11 subjects). Fold change in gMFI over DN were depicted. * P <0.05, ** P <0.01 (one-way ANOVA with Tukey’s multiple comparisons test). gMFI, geometric mean fluorescence intensity. (b) Expression level of ABC between CXCR3 − and CXCR3 + ex vivo Tregs from healthy controls. Representative histogram (left) and summary of results (n=7 subjects) (right). * P <0.05 (two-tailed paired Student’s t -test). (c) Gene expression of Wnt/ β-catenin signaling target genes ( AXIN2 and TCF7 ) assessed by RNA-seq. * P <0.05, ** P <0.01 (one-way ANOVA with Tukey’s multiple comparisons test). (d) Relative expression level of ABC on Tregs stimulated with anti-CD3 and anti-CD28 for 4 days, followed by 4 h PMA/iomomycin stimulation and intracellular cytokine staining for IFNγ and IL-10 (n=12 subjects). Fold change in gMFI over DN were depicted. * P <0.05, ** P <0.01, *** P <0.001 (one-way ANOVA with Tukey’s multiple comparisons test). (e) Expression level of β-catenin on Tregs stimulated with anti-CD3 and anti-CD28 in the presence (Th1) or absence (Th0) of <t>IL-12</t> for 4 days. β-catenin level was determined directly by intracellular staining (left) (n=9 subjects) and β-catenin level on IFNγ positive/negative Treg populations was determined after 4 h PMA/iomomycin stimulation (middle) (n=4 subjects). Representative histogram for β-catenin expression was shown (right). * P <0.05 (two-tailed unpaired Student’s t -test). (f, g) Frequency of IFNγ and IL-10 positive cell number ( f ) and gene expression of IFNG and IL10 by qPCR ( g ). Tregs were stimulated with anti-CD3 and anti-CD28 in the presence of Wnt/β-catenin signaling inhibitor PKF115-584 (PKF), IL-12 (Th1), or IL-12 and PKF115-584 (Th1 + PKF) (n=4 subjects) * P <0.05, ** P <0.01, *** P <0.001 (one-way ANOVA with Tukey’s multiple comparisons test). (h, i) Relative frequency of IFNγ and IL-10 positive cell number (fold of scramble shRNA/control condition) ( h ) and gene expression of IFNG and IL10 by qPCR ( i ). Tregs were transduced with a non-targeted shRNA or a CTNNB1 shRNA and cultured in Th0 or Th1 condition for 5 days ( h ; n=7 subjects, i ; n=5 subjects). * P <0.05, ** P <0.01 (one-way ANOVA with Tukey’s multiple comparisons test). Data are representative of two experiments (e (middle), and f) or are from more than three experiments.
Infinium Genotyping Assays, supplied by INFINIUM Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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90
NimbleGen Systems GmbH hybridization system
(a) Relative expression level of Active β-catenin (ABC) on ex vivo Treg subpopulations analyzed by flow cytometry (n=11 subjects). Fold change in gMFI over DN were depicted. * P <0.05, ** P <0.01 (one-way ANOVA with Tukey’s multiple comparisons test). gMFI, geometric mean fluorescence intensity. (b) Expression level of ABC between CXCR3 − and CXCR3 + ex vivo Tregs from healthy controls. Representative histogram (left) and summary of results (n=7 subjects) (right). * P <0.05 (two-tailed paired Student’s t -test). (c) Gene expression of Wnt/ β-catenin signaling target genes ( AXIN2 and TCF7 ) assessed by RNA-seq. * P <0.05, ** P <0.01 (one-way ANOVA with Tukey’s multiple comparisons test). (d) Relative expression level of ABC on Tregs stimulated with anti-CD3 and anti-CD28 for 4 days, followed by 4 h PMA/iomomycin stimulation and intracellular cytokine staining for IFNγ and IL-10 (n=12 subjects). Fold change in gMFI over DN were depicted. * P <0.05, ** P <0.01, *** P <0.001 (one-way ANOVA with Tukey’s multiple comparisons test). (e) Expression level of β-catenin on Tregs stimulated with anti-CD3 and anti-CD28 in the presence (Th1) or absence (Th0) of <t>IL-12</t> for 4 days. β-catenin level was determined directly by intracellular staining (left) (n=9 subjects) and β-catenin level on IFNγ positive/negative Treg populations was determined after 4 h PMA/iomomycin stimulation (middle) (n=4 subjects). Representative histogram for β-catenin expression was shown (right). * P <0.05 (two-tailed unpaired Student’s t -test). (f, g) Frequency of IFNγ and IL-10 positive cell number ( f ) and gene expression of IFNG and IL10 by qPCR ( g ). Tregs were stimulated with anti-CD3 and anti-CD28 in the presence of Wnt/β-catenin signaling inhibitor PKF115-584 (PKF), IL-12 (Th1), or IL-12 and PKF115-584 (Th1 + PKF) (n=4 subjects) * P <0.05, ** P <0.01, *** P <0.001 (one-way ANOVA with Tukey’s multiple comparisons test). (h, i) Relative frequency of IFNγ and IL-10 positive cell number (fold of scramble shRNA/control condition) ( h ) and gene expression of IFNG and IL10 by qPCR ( i ). Tregs were transduced with a non-targeted shRNA or a CTNNB1 shRNA and cultured in Th0 or Th1 condition for 5 days ( h ; n=7 subjects, i ; n=5 subjects). * P <0.05, ** P <0.01 (one-way ANOVA with Tukey’s multiple comparisons test). Data are representative of two experiments (e (middle), and f) or are from more than three experiments.
Hybridization System, supplied by NimbleGen Systems GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
hybridization system - by Bioz Stars, 2026-09
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90
LC Sciences microarray
(a) Relative expression level of Active β-catenin (ABC) on ex vivo Treg subpopulations analyzed by flow cytometry (n=11 subjects). Fold change in gMFI over DN were depicted. * P <0.05, ** P <0.01 (one-way ANOVA with Tukey’s multiple comparisons test). gMFI, geometric mean fluorescence intensity. (b) Expression level of ABC between CXCR3 − and CXCR3 + ex vivo Tregs from healthy controls. Representative histogram (left) and summary of results (n=7 subjects) (right). * P <0.05 (two-tailed paired Student’s t -test). (c) Gene expression of Wnt/ β-catenin signaling target genes ( AXIN2 and TCF7 ) assessed by RNA-seq. * P <0.05, ** P <0.01 (one-way ANOVA with Tukey’s multiple comparisons test). (d) Relative expression level of ABC on Tregs stimulated with anti-CD3 and anti-CD28 for 4 days, followed by 4 h PMA/iomomycin stimulation and intracellular cytokine staining for IFNγ and IL-10 (n=12 subjects). Fold change in gMFI over DN were depicted. * P <0.05, ** P <0.01, *** P <0.001 (one-way ANOVA with Tukey’s multiple comparisons test). (e) Expression level of β-catenin on Tregs stimulated with anti-CD3 and anti-CD28 in the presence (Th1) or absence (Th0) of <t>IL-12</t> for 4 days. β-catenin level was determined directly by intracellular staining (left) (n=9 subjects) and β-catenin level on IFNγ positive/negative Treg populations was determined after 4 h PMA/iomomycin stimulation (middle) (n=4 subjects). Representative histogram for β-catenin expression was shown (right). * P <0.05 (two-tailed unpaired Student’s t -test). (f, g) Frequency of IFNγ and IL-10 positive cell number ( f ) and gene expression of IFNG and IL10 by qPCR ( g ). Tregs were stimulated with anti-CD3 and anti-CD28 in the presence of Wnt/β-catenin signaling inhibitor PKF115-584 (PKF), IL-12 (Th1), or IL-12 and PKF115-584 (Th1 + PKF) (n=4 subjects) * P <0.05, ** P <0.01, *** P <0.001 (one-way ANOVA with Tukey’s multiple comparisons test). (h, i) Relative frequency of IFNγ and IL-10 positive cell number (fold of scramble shRNA/control condition) ( h ) and gene expression of IFNG and IL10 by qPCR ( i ). Tregs were transduced with a non-targeted shRNA or a CTNNB1 shRNA and cultured in Th0 or Th1 condition for 5 days ( h ; n=7 subjects, i ; n=5 subjects). * P <0.05, ** P <0.01 (one-way ANOVA with Tukey’s multiple comparisons test). Data are representative of two experiments (e (middle), and f) or are from more than three experiments.
Microarray, supplied by LC Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
microarray - by Bioz Stars, 2026-09
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90
PamGene International B.V pamstation 12
(a) Relative expression level of Active β-catenin (ABC) on ex vivo Treg subpopulations analyzed by flow cytometry (n=11 subjects). Fold change in gMFI over DN were depicted. * P <0.05, ** P <0.01 (one-way ANOVA with Tukey’s multiple comparisons test). gMFI, geometric mean fluorescence intensity. (b) Expression level of ABC between CXCR3 − and CXCR3 + ex vivo Tregs from healthy controls. Representative histogram (left) and summary of results (n=7 subjects) (right). * P <0.05 (two-tailed paired Student’s t -test). (c) Gene expression of Wnt/ β-catenin signaling target genes ( AXIN2 and TCF7 ) assessed by RNA-seq. * P <0.05, ** P <0.01 (one-way ANOVA with Tukey’s multiple comparisons test). (d) Relative expression level of ABC on Tregs stimulated with anti-CD3 and anti-CD28 for 4 days, followed by 4 h PMA/iomomycin stimulation and intracellular cytokine staining for IFNγ and IL-10 (n=12 subjects). Fold change in gMFI over DN were depicted. * P <0.05, ** P <0.01, *** P <0.001 (one-way ANOVA with Tukey’s multiple comparisons test). (e) Expression level of β-catenin on Tregs stimulated with anti-CD3 and anti-CD28 in the presence (Th1) or absence (Th0) of <t>IL-12</t> for 4 days. β-catenin level was determined directly by intracellular staining (left) (n=9 subjects) and β-catenin level on IFNγ positive/negative Treg populations was determined after 4 h PMA/iomomycin stimulation (middle) (n=4 subjects). Representative histogram for β-catenin expression was shown (right). * P <0.05 (two-tailed unpaired Student’s t -test). (f, g) Frequency of IFNγ and IL-10 positive cell number ( f ) and gene expression of IFNG and IL10 by qPCR ( g ). Tregs were stimulated with anti-CD3 and anti-CD28 in the presence of Wnt/β-catenin signaling inhibitor PKF115-584 (PKF), IL-12 (Th1), or IL-12 and PKF115-584 (Th1 + PKF) (n=4 subjects) * P <0.05, ** P <0.01, *** P <0.001 (one-way ANOVA with Tukey’s multiple comparisons test). (h, i) Relative frequency of IFNγ and IL-10 positive cell number (fold of scramble shRNA/control condition) ( h ) and gene expression of IFNG and IL10 by qPCR ( i ). Tregs were transduced with a non-targeted shRNA or a CTNNB1 shRNA and cultured in Th0 or Th1 condition for 5 days ( h ; n=7 subjects, i ; n=5 subjects). * P <0.05, ** P <0.01 (one-way ANOVA with Tukey’s multiple comparisons test). Data are representative of two experiments (e (middle), and f) or are from more than three experiments.
Pamstation 12, supplied by PamGene International B.V, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
pamstation 12 - by Bioz Stars, 2026-09
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90
Ocimum Biosolutions e. coli glass-slide microarrays
(a) Relative expression level of Active β-catenin (ABC) on ex vivo Treg subpopulations analyzed by flow cytometry (n=11 subjects). Fold change in gMFI over DN were depicted. * P <0.05, ** P <0.01 (one-way ANOVA with Tukey’s multiple comparisons test). gMFI, geometric mean fluorescence intensity. (b) Expression level of ABC between CXCR3 − and CXCR3 + ex vivo Tregs from healthy controls. Representative histogram (left) and summary of results (n=7 subjects) (right). * P <0.05 (two-tailed paired Student’s t -test). (c) Gene expression of Wnt/ β-catenin signaling target genes ( AXIN2 and TCF7 ) assessed by RNA-seq. * P <0.05, ** P <0.01 (one-way ANOVA with Tukey’s multiple comparisons test). (d) Relative expression level of ABC on Tregs stimulated with anti-CD3 and anti-CD28 for 4 days, followed by 4 h PMA/iomomycin stimulation and intracellular cytokine staining for IFNγ and IL-10 (n=12 subjects). Fold change in gMFI over DN were depicted. * P <0.05, ** P <0.01, *** P <0.001 (one-way ANOVA with Tukey’s multiple comparisons test). (e) Expression level of β-catenin on Tregs stimulated with anti-CD3 and anti-CD28 in the presence (Th1) or absence (Th0) of <t>IL-12</t> for 4 days. β-catenin level was determined directly by intracellular staining (left) (n=9 subjects) and β-catenin level on IFNγ positive/negative Treg populations was determined after 4 h PMA/iomomycin stimulation (middle) (n=4 subjects). Representative histogram for β-catenin expression was shown (right). * P <0.05 (two-tailed unpaired Student’s t -test). (f, g) Frequency of IFNγ and IL-10 positive cell number ( f ) and gene expression of IFNG and IL10 by qPCR ( g ). Tregs were stimulated with anti-CD3 and anti-CD28 in the presence of Wnt/β-catenin signaling inhibitor PKF115-584 (PKF), IL-12 (Th1), or IL-12 and PKF115-584 (Th1 + PKF) (n=4 subjects) * P <0.05, ** P <0.01, *** P <0.001 (one-way ANOVA with Tukey’s multiple comparisons test). (h, i) Relative frequency of IFNγ and IL-10 positive cell number (fold of scramble shRNA/control condition) ( h ) and gene expression of IFNG and IL10 by qPCR ( i ). Tregs were transduced with a non-targeted shRNA or a CTNNB1 shRNA and cultured in Th0 or Th1 condition for 5 days ( h ; n=7 subjects, i ; n=5 subjects). * P <0.05, ** P <0.01 (one-way ANOVA with Tukey’s multiple comparisons test). Data are representative of two experiments (e (middle), and f) or are from more than three experiments.
E. Coli Glass Slide Microarrays, supplied by Ocimum Biosolutions, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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LC Sciences mirna microarray
Characteristics of included <t> miRNA </t> profiling studies
Mirna Microarray, supplied by LC Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Randox rx monaco analyser (benchtop), rx monaco analyser (with stand), rx monaco pc
Characteristics of included <t> miRNA </t> profiling studies
Rx Monaco Analyser (Benchtop), Rx Monaco Analyser (With Stand), Rx Monaco Pc, supplied by Randox, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Randox rx misano analyser, rx misano analyser (with printer), rx misano analyser (honey analysis), rx misano analyser with printer (honey analysis), rx misano incubator
Characteristics of included <t> miRNA </t> profiling studies
Rx Misano Analyser, Rx Misano Analyser (With Printer), Rx Misano Analyser (Honey Analysis), Rx Misano Analyser With Printer (Honey Analysis), Rx Misano Incubator, supplied by Randox, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Randox rx modena analsyer, rx modena pc, printer
Characteristics of included <t> miRNA </t> profiling studies
Rx Modena Analsyer, Rx Modena Pc, Printer, supplied by Randox, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(a) Relative expression level of Active β-catenin (ABC) on ex vivo Treg subpopulations analyzed by flow cytometry (n=11 subjects). Fold change in gMFI over DN were depicted. * P <0.05, ** P <0.01 (one-way ANOVA with Tukey’s multiple comparisons test). gMFI, geometric mean fluorescence intensity. (b) Expression level of ABC between CXCR3 − and CXCR3 + ex vivo Tregs from healthy controls. Representative histogram (left) and summary of results (n=7 subjects) (right). * P <0.05 (two-tailed paired Student’s t -test). (c) Gene expression of Wnt/ β-catenin signaling target genes ( AXIN2 and TCF7 ) assessed by RNA-seq. * P <0.05, ** P <0.01 (one-way ANOVA with Tukey’s multiple comparisons test). (d) Relative expression level of ABC on Tregs stimulated with anti-CD3 and anti-CD28 for 4 days, followed by 4 h PMA/iomomycin stimulation and intracellular cytokine staining for IFNγ and IL-10 (n=12 subjects). Fold change in gMFI over DN were depicted. * P <0.05, ** P <0.01, *** P <0.001 (one-way ANOVA with Tukey’s multiple comparisons test). (e) Expression level of β-catenin on Tregs stimulated with anti-CD3 and anti-CD28 in the presence (Th1) or absence (Th0) of IL-12 for 4 days. β-catenin level was determined directly by intracellular staining (left) (n=9 subjects) and β-catenin level on IFNγ positive/negative Treg populations was determined after 4 h PMA/iomomycin stimulation (middle) (n=4 subjects). Representative histogram for β-catenin expression was shown (right). * P <0.05 (two-tailed unpaired Student’s t -test). (f, g) Frequency of IFNγ and IL-10 positive cell number ( f ) and gene expression of IFNG and IL10 by qPCR ( g ). Tregs were stimulated with anti-CD3 and anti-CD28 in the presence of Wnt/β-catenin signaling inhibitor PKF115-584 (PKF), IL-12 (Th1), or IL-12 and PKF115-584 (Th1 + PKF) (n=4 subjects) * P <0.05, ** P <0.01, *** P <0.001 (one-way ANOVA with Tukey’s multiple comparisons test). (h, i) Relative frequency of IFNγ and IL-10 positive cell number (fold of scramble shRNA/control condition) ( h ) and gene expression of IFNG and IL10 by qPCR ( i ). Tregs were transduced with a non-targeted shRNA or a CTNNB1 shRNA and cultured in Th0 or Th1 condition for 5 days ( h ; n=7 subjects, i ; n=5 subjects). * P <0.05, ** P <0.01 (one-way ANOVA with Tukey’s multiple comparisons test). Data are representative of two experiments (e (middle), and f) or are from more than three experiments.

Journal: bioRxiv

Article Title: PTGER2-β-Catenin Axis Links High Salt Environments to Autoimmunity by Balancing IFNγ and IL-10 in FoxP3 + Regulatory T cells

doi: 10.1101/379453

Figure Lengend Snippet: (a) Relative expression level of Active β-catenin (ABC) on ex vivo Treg subpopulations analyzed by flow cytometry (n=11 subjects). Fold change in gMFI over DN were depicted. * P <0.05, ** P <0.01 (one-way ANOVA with Tukey’s multiple comparisons test). gMFI, geometric mean fluorescence intensity. (b) Expression level of ABC between CXCR3 − and CXCR3 + ex vivo Tregs from healthy controls. Representative histogram (left) and summary of results (n=7 subjects) (right). * P <0.05 (two-tailed paired Student’s t -test). (c) Gene expression of Wnt/ β-catenin signaling target genes ( AXIN2 and TCF7 ) assessed by RNA-seq. * P <0.05, ** P <0.01 (one-way ANOVA with Tukey’s multiple comparisons test). (d) Relative expression level of ABC on Tregs stimulated with anti-CD3 and anti-CD28 for 4 days, followed by 4 h PMA/iomomycin stimulation and intracellular cytokine staining for IFNγ and IL-10 (n=12 subjects). Fold change in gMFI over DN were depicted. * P <0.05, ** P <0.01, *** P <0.001 (one-way ANOVA with Tukey’s multiple comparisons test). (e) Expression level of β-catenin on Tregs stimulated with anti-CD3 and anti-CD28 in the presence (Th1) or absence (Th0) of IL-12 for 4 days. β-catenin level was determined directly by intracellular staining (left) (n=9 subjects) and β-catenin level on IFNγ positive/negative Treg populations was determined after 4 h PMA/iomomycin stimulation (middle) (n=4 subjects). Representative histogram for β-catenin expression was shown (right). * P <0.05 (two-tailed unpaired Student’s t -test). (f, g) Frequency of IFNγ and IL-10 positive cell number ( f ) and gene expression of IFNG and IL10 by qPCR ( g ). Tregs were stimulated with anti-CD3 and anti-CD28 in the presence of Wnt/β-catenin signaling inhibitor PKF115-584 (PKF), IL-12 (Th1), or IL-12 and PKF115-584 (Th1 + PKF) (n=4 subjects) * P <0.05, ** P <0.01, *** P <0.001 (one-way ANOVA with Tukey’s multiple comparisons test). (h, i) Relative frequency of IFNγ and IL-10 positive cell number (fold of scramble shRNA/control condition) ( h ) and gene expression of IFNG and IL10 by qPCR ( i ). Tregs were transduced with a non-targeted shRNA or a CTNNB1 shRNA and cultured in Th0 or Th1 condition for 5 days ( h ; n=7 subjects, i ; n=5 subjects). * P <0.05, ** P <0.01 (one-way ANOVA with Tukey’s multiple comparisons test). Data are representative of two experiments (e (middle), and f) or are from more than three experiments.

Article Snippet: Th1-Tregs induced with human recombinant IL-12 (20 ng/ml) (R&D).

Techniques: Expressing, Ex Vivo, Flow Cytometry, Fluorescence, Two Tailed Test, RNA Sequencing Assay, Staining, shRNA, Transduction, Cell Culture

(a) AXIN2 and TCF7 mRNA expression in IFNγ + and IFNγ − human Treg populations assessed by DNA microarray (n=8 subjects) . * P <0.05 (two-tailed paired Student’s t -test). (b) CTNNB1 gene expression on Tregs transduced with a non-targeted shRNA or a CTNNB1 shRNA and cultured for 5 days (n=10 subjects). *** P <0.001 (two-tailed unpaired Student’s t -test). (c) Flow cytometric analysis of TCF1 expression on ex vivo Treg subpopulations relative to DN (n=8 subjects). * P <0.05, ** P <0.01 (one-way ANOVA with Tukey’s multiple comparisons test). (d) Frequency of IFNγ and IL-10 positive cell number. Tregs were stimulated with anti-CD3 and anti-CD28 in the presence of SGK1 inhibitor GSK650394 (SGK1-i), IL-12 (Th1), or IL-12 and GSK650394 (Th1 + SGK1-i) (n=6 subjects) *** P <0.001 (one-way ANOVA with Tukey’s multiple comparisons test).

Journal: bioRxiv

Article Title: PTGER2-β-Catenin Axis Links High Salt Environments to Autoimmunity by Balancing IFNγ and IL-10 in FoxP3 + Regulatory T cells

doi: 10.1101/379453

Figure Lengend Snippet: (a) AXIN2 and TCF7 mRNA expression in IFNγ + and IFNγ − human Treg populations assessed by DNA microarray (n=8 subjects) . * P <0.05 (two-tailed paired Student’s t -test). (b) CTNNB1 gene expression on Tregs transduced with a non-targeted shRNA or a CTNNB1 shRNA and cultured for 5 days (n=10 subjects). *** P <0.001 (two-tailed unpaired Student’s t -test). (c) Flow cytometric analysis of TCF1 expression on ex vivo Treg subpopulations relative to DN (n=8 subjects). * P <0.05, ** P <0.01 (one-way ANOVA with Tukey’s multiple comparisons test). (d) Frequency of IFNγ and IL-10 positive cell number. Tregs were stimulated with anti-CD3 and anti-CD28 in the presence of SGK1 inhibitor GSK650394 (SGK1-i), IL-12 (Th1), or IL-12 and GSK650394 (Th1 + SGK1-i) (n=6 subjects) *** P <0.001 (one-way ANOVA with Tukey’s multiple comparisons test).

Article Snippet: Th1-Tregs induced with human recombinant IL-12 (20 ng/ml) (R&D).

Techniques: Expressing, Microarray, Two Tailed Test, Transduction, shRNA, Cell Culture, Ex Vivo

Characteristics of included  miRNA  profiling studies

Journal: Bioscience Reports

Article Title: Differentially expressed circulating miRNAs in postmenopausal osteoporosis: a meta-analysis

doi: 10.1042/BSR20190667

Figure Lengend Snippet: Characteristics of included miRNA profiling studies

Article Snippet: 12 , Chen C , 2013 , China , Blood , 20 (10/10) , miRNA microarray, LC Sciences , 721 , 7 , 3 , 4 , P <0.05.

Techniques: Clinical Proteomics, Microarray, Sequencing

* indicates miRNAs reported in more than one study, # indicates miRNAs reported to be dysregulated in both directions.

Journal: Bioscience Reports

Article Title: Differentially expressed circulating miRNAs in postmenopausal osteoporosis: a meta-analysis

doi: 10.1042/BSR20190667

Figure Lengend Snippet: * indicates miRNAs reported in more than one study, # indicates miRNAs reported to be dysregulated in both directions.

Article Snippet: 12 , Chen C , 2013 , China , Blood , 20 (10/10) , miRNA microarray, LC Sciences , 721 , 7 , 3 , 4 , P <0.05.

Techniques: